sacB [2017-11-14 13:05:36]

levansucrase

Locus: BSU34450

Isoelectric point: 6.18

Molecular weight: 52.80 kDa

Protein length: 473 aa

Gene length: 1419 bp

Function: utilization of sucrose, production of levan

Product: levansucrase

Essential: no

E.C.: 2.4.1.10

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of sucrose
• Groups of genes → Secreted proteins

Gene

Coordinates: 3,536,012 → 3,537,433

The protein

Catalyzed reaction/ biological activity

• Sucrose (6)-beta-D-fructofuranosyl-(2→)(n) alpha-D-glucopyranoside = glucose (6)-beta-D-fructofuranosyl-(2→)(n 1) alpha-D-glucopyranoside (according to Swiss-Prot)

Protein family

• glycosyl hydrolase 68 family (according to Swiss-Prot)

Structure

• 1PT2 (complex with sucrose), 1OYG

Localization

• secreted (according to Swiss-Prot), extracellular (signal peptide) PubMed

Expression and Regulation

Operons

• Genes: sacB-levB
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • DegU: activation, DegU-P, PubMed, in DegU regulon
  • SacY: antitermination, /antitermination via binding to a RNA switch, in SacY regulon

  Regulation

  • the RNA switch RNA is degraded by RNase Y PubMed

Biological materials

Mutant

• BKE34450 (ΔsacB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCGTTCATGTCTCCTTTT, downstream forward: _UP4_TAAAAACGCAAAAGAAAATG
• BKK34450 (ΔsacB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCGTTCATGTCTCCTTTT, downstream forward: _UP4_TAAAAACGCAAAAGAAAATG

LacZ fusion

• pGP564 (in pAC7), a series of RAT mutations in pAC7, all available in Stülke lab

References

Reviews

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Original publications

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